sorry... i'm here to grumble again...
seems like not many good things happen to me
ppl who are not interested in the details my lab experiments, you can basically skip this blog entry.

this time is abt PAUL.
PAUL is annoying!
he's old and dodgy, but my supervisor still likes him.

twice already, becoz of paul, i had to go back to the lab all the way, once from home 7.55pm tram, the other time was just now, 8 something, from the city and with my aunt who came visit me here in melb.

oh by the way, PAUL is the name of a PCR machine in our lab
we have 3 PCR machines, and they all have names - CHAD, BRAD and PAUL
CHAD and BRAD are the newest ones we have.
PAUL is an older model so there are some settings that have to be done manually
and he can't hold completed PCR at 4 degrees, instead, sometimes they'll be at 24, 25 C or even 29, 30C like just now!
high temperature is bad for DNA (oh... PCR is a reaction to make more DNA from a minimum amount of DNA as starting material)

i was right at the first time, luckily i went back to check the settings
but this time, i was too paranoid coz i had the settings right before i left at 5.30pm but when i went back just now, it was at 30C!

i've been repeating my PCR for so many times already and they aren't working until this afternoon.
so if this one is screwed (which i'll know on monday), i'll blame PAUL totally!

I did explain to my supervisor abt my concern with using Paul, but he said room temp is ok for DNA, that's considering 24C as room temp, but now it's 30C!!!
luckily i didn't leave it for overnight (which i planned to go back to the lab only on tmr morning).